Molecular Cloning: A Laboratory Manual by Joseph Sambrook
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Molecular Cloning: A Laboratory Manual, Volume 1, 2, 3
Joseph Frank Sambrook born 1 March in Liverpool , England is a British molecular biologist known for his studies of DNA oncoviruses and the molecular biology of normal and cancerous cells. He resides in Melbourne, Australia with his wife and daughter. In he was hired by James D. Watson has been reported to say this was the best hiring decision he ever made. Joe was responsible for creating a combative creative environment at CSHL that fomented discovery. Sambrook is best known for his studies on DNA tumor viruses and the molecular biology of normal and neoplastic cells. His Tumour Virus Group at Cold Spring Harbor identified and mapped all of the major genes of adenoviruses and SV40 , determined their transcriptional control in infected and transformed cells, and elucidated the mechanism of integration of these viruses into the genome of the host cell.
Click to Enlarge. Download Preface and Contents Download Index. Free shipping offer applies to direct website purchases by individual U. This title also available in: Paperback three-volume set. Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years. No other manual has been so popular, or so influential. Molecular Cloning, Fourth Edition, by the celebrated founding author Joe Sambrook and new co-author, the distinguished HHMI investigator Michael Green, preserves the highly praised detail and clarity of previous editions and includes specific chapters and protocols commissioned for the book from expert practitioners at Yale, U Mass, Rockefeller University, Texas Tech, Cold Spring Harbor Laboratory, Washington University, and other leading institutions.
The first two editions of this manual have been mainstays of molecular biology for nearly twenty years, with an unrivalled reputation for reliability, accuracy, and clarity. In this new edition, authors Joseph Sambrook and David Russell have completely updated the book, revising every protocol and adding a mass of new material, to broaden its scope and maintain its unbeatable value for studies in genetics, molecular cell biology, developmental biology, microbiology, neuroscience, and immunology. The opening chapters describe essential techniques, some well—established, some new, that are used every day in the best laboratories for isolating, analyzing and cloning DNA molecules, both large and small. The concluding chapters deal with methods to screen expression libraries, express cloned genes in both prokaryotes and eukaryotic cells, analyze transcripts and proteins, and detect protein—protein interactions. The Appendix is a compendium of reagents, vectors, media, technical suppliers, kits, electronic resources and other essential information. As in earlier editions, this is the only manual that explains how to achieve success in cloning and provides a wealth of information about why techniques work, how they were first developed, and how they have evolved.